Hi, here is the link:

https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Datasheet/pbs1dat.pdf

Based on this, temperature is also important. Good luck!

Hi there,

I think the following link should help you:

http://clymer.altervista.org/buffers/phos2.html

btw, 50mM phosphate buffer is not actually PBS... See the following link:

https://en.wikipedia.org/wiki/Phosphate-buffered_saline

Refer to Methods in Enzymology Vol1

http://serge.engi.tripod.com/MolBio/Buffer_cal.html

http://cshprotocols.cshlp.org/content/2006/1/pdb.rec8303.full

In the attach file you can find good information about

phosphate buffer solution preparations