How can I prepare 20 mM Tris-HCL, pH=8 to a concentration of 01-1.0 mg/mL?

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Sabine Strehl

Here is a protocol for making a Tris-HCL pH=8 stock solution

https://toptipbio.com/tris-hcl-recipe/

Dilute it to 20 mM working solution using this calculator https://functionalbio.com/resources/dilution-calculator/dilution-calculator-molarity/

Chen Jingwen

Hi,

You may use the free web tool to calculate

https://www.alfa-chemistry.com/solution-dilution-calculator.htm

https://www.alfa-chemistry.com/mass-molarity-calculator.htm