Cemre Kirşan

3 Questions 1 Answers 0 Followers

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Why my primary cultured monocytes die?

I'm doing isolation and culture of human monocytes from healthy subjects. I'm obtaining PBMCs from whole blood and purifying monocytes using density gradient centrifugation method. After...

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How can I prepare 20 mM Tris-HCL, pH=8 to a concentration of 01-1.0 mg/mL?

I want to to reconstitute the lyophilized M-CSF in Tris-HCL

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What should I use to remove cells prior to RNA isolation in primary macrophages cell culture?

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