What could be the reason of no transformation using a ligation mixture?

Recently, I have been working with some genes contained in pBlueScript II SK (+) and that contain XhoI and XbaI restriction sites. I would like to clone them in PET28a. For instance, the...

22 June 2020 3,474 5 View

What factors influence user acceptance of affective technologies?

Affective technologies are the interfaces concerning the emotional artificial intelligence branch known as affective computing (Picard, 1997). Applications such as facial emotion recognition...

08 May 2020 2,973 9 View

Are there zinc-CYS parameters in CHARMM files? of has anyone generated these parameters?

06 March 2020 5,939 2 View

How do I run a successful digestion for a large plasmid with GC Repeats, hairpins, and potentially other secondary structure?

I am doing some cloning and am having some issues with both sequencing my 22kb plasmid and running digestions with it. I sent my plasmid in for sequencing and got terrible results, but...

02 July 2019 1,506 3 View

How to assess machine learning tools in eCognition Developer version 9.3.2?

How to assess machine learning classification tools in the latest release of eCognition Developer 9.3.2.

17 September 2018 8,963 1 View

I am looking for a simple basic description of a system within systems theory used to describe an organisation?

I have studied systems theory in the more complex mathematical version and have a handout in Dutch describing an organisation as a system with the primary process and two kinds of feedback loops....

15 April 2018 6,746 2 View

Preparation of spheroids for microscopic analysis

I'm using HepG2 to form 3D HepG2 aggregates with alginate, to confocal microscopy analyzes. However, I have difficulty to maintaining the spheroids after fixation and all procedures of incubations...

24 July 2017 478 1 View

Does anyone work with esophageal cell line immortalized with hTERT ?

22 June 2016 3,575 3 View

Why does an H&E stained slide that showed the presence of calcium does not stain positive for von Kossa?

22 February 2015 9,571 4 View

ERK5 Ab to ChiP:

Does anyone use Anti ERK5 to Chromatin Immunoprecipitation assay (ChiP)? I want to start this protocol but dont know where I can find this Ab.

14 May 2013 2,365 1 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View