I am raw to this area. I would like to know how to generate a primer to a specific gene from a specific bacterium or virus, like Escherichia coli or HPV, for instance. Does the organism has to be one which genome is already sequenced? If yes, can I obtain a primer for a specific gene that was not used elsewhere yet? Does it have comercial rights if someone has already identified simultaneously, from separate labs in the world, the same gene I want to identify?
I thought about going through reverse genetics, RFLP, VNTR and SNP. Am I in the right path?
Hi João Carmo
Simply, you can type your gene in Genbank website https://www.ncbi.nlm.nih.gov/genbank/
and check if the sequence available or not. If you get the sequence, you can search for primers using primer design software e.g. https://www.ncbi.nlm.nih.gov/tools/primer-blast/
You can also used published primer and just cite in your work.
Hello,
If the specific organism your interested does not yet have a published genome then what I would recommend is to look at the gene sequences from related organisms. If you find conserved areas you can begin to design primers based on those. This may require some trial and error, as perhaps a sequence conserved in the relatives is not actualy conserved in your target organism. Typically when I design new primers I order a few that I think have a chance of working, test them all, and then I either have one or two good candidates or I have to start over.
There's nothing wrong with using other people's primers as long as you cite them, as Ahmed mentioned in his answer.
Hi everyone,
Check out this one stop website that makes searching information related to protein,, DNA, RNA and more, easier and quicker!
www.biodeets.com
Thanks!
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