Dear Mahesh

Thanks,

I am interesting in dealing with saliva sample before performing RIA or ELISA

some researches menstioned to add protease inhibitor cocktail, others do not..

so I am confused about the best way to handle the samples

Dear Maysaa,

You can use this kit from salimetrics to detect IL-6:

http://www.salimetrics.com/assay-kit/interleukin-6-il-6-salivary-immunoassay-kit-elisa-eia

Also, have a look at this research paper:

http://www.ncbi.nlm.nih.gov/m/pubmed/15313862/

I hope this will be helpful.