How do you expect the film to be toxic? What delivery mechanisms do you want to test ... air, ingestion, through the skin ...? What type of toxicity response do you want to test against ... sickness, allergic reaction, death ... , for what duration ... short term or long term ..., and at what exposure level ... low dose, high dose, kill dose ... ?
The question is complex, as you can perhaps appreciate from this. A simple yet perhaps cynical answer also to illustrate the complexity is ...
You can measure the toxicity of a CdS thin film by this experiment:
* chop up your sample
* eat some of it
* see how long you stay alive
* if you don't die, eat some more
* repeat the above steps until you die
* write a publication to us on your results
So, you tell me ... How do you want to measure the toxicity of a CdS thin film?
You can do the following assays at various concentrations (CdS)
In vitro toxicity assays
1. MTT Assay (using normal cells i.e. Murine macrophage cells (RAW cells))
2. Comet Assay (using human lymphocytes)
3. Genotoxicity assay (using microbial cells)
In vivo toxicity assay (using Rat or Mouse or Rabbit)
1. Acute toxicity study
2. Chronic toxicity study
i accept Jeffrey Weimer and dr. rajaram suggestion.. and also i will suggested trypan blue method for cell viability test . it is simple method (0.4%-Trypan blue) to do toxicity an in vitro (Cell lines) or you will try lymphocytes.
Dear Jeffrey,
I have a same problem too. In brief, we are coating various types of binary, ternary, and nanocomposite semiconductor metal oxide on different kind of organic and inorganic substrates using PVD systems. But, I have trouble finding suitable procedure for evaluation of antioxidant, toxicity both in in vivo and in vitro models. My main question is, how can measure these assays for thin films coated on substrate??
Thanks,
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