How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

How to count the number of figure when reusing figure??

Dear all I'm preparing a review paper and confused about terms for reusing figure in other papers... Can you please help me for 2 question..?? (1) As i know, the cost of reusing figure are...

28 December 2020 1,515 3 View

Any suggestion for conductive paste for PDMS or Ecoflex for electrode?

Dear colleagues, I am trying to make a sturdy electrode on a flexible polymer substrate (PDMS or Ecoflex). I have used a silver paste, but once cured, it peels off very easily. the cured silver...

14 December 2020 4,236 3 View

Can I autoclave d-mannose?

Hello everyone, I was wondering whether I should autoclave or filter d-mannose solution. In our laboratory, we autoclave glucose separately when we make YPD media for yeast culture. So, I am...

08 October 2020 7,068 1 View

Is there any general way to make polymer hydrophobic?

Now I try to make hydrophobic pillar array using Resin 3D printing (Formlab's 'Surgical Guide Resin'). But actually I don't know exactly what the resin is made of. But as we know it is the mixture...

05 July 2020 3,860 5 View

Does topology from ATB support FF other than Gromos?

I am new to MD / gromacs and I am trying to use MD not for biological system but for generic materials science. Specifically, I am trying to simulate the interaction between hydrophobic molecules...

07 June 2020 8,989 2 View

How can I use a molecule from ATB in Gromacs?

I am new to MD / gromacs and I am trying to use MD not for biological system but for generic materials science. Specifically, I am trying to simulate the interaction between hydrophobic molecules...

06 June 2020 2,112 4 View

I want measure intracellular pH change in sperm with BCECF-AM. It requires pH standard?

This is my first time to try measure intracellular pH but it is confusing about protocol. I have BCECF/AM (216254, CAS 117464-70-7, Sigma) and i understood protocol briefly as below. 1. Load...

04 June 2020 6,392 6 View

Tips on interpreting cell morphology and counting mouse primary aortic smooth muscle cells?

Hello, I recently started culturing mouse primary aortic smooth muscle cells (purchased from Cell Biologics) with the media supplied by the same company (5% FBS and a whole lot of other stuff)....

19 March 2020 9,781 3 View

Does anyone know about analysis of 3D mapping and depth profile for wooden object?

I have 1cm cubic size wood samples. inside of wood, there are S and S-organic, inorganic compounds randomly. I'd like to figure out the quantities of S distribution non-destructively. First, I...

20 February 2020 9,303 2 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

How to quantify carbon deposition on CuZnAl catalyst?

Dear All, I want to quantify carbon deposition on the surface of the CuZnAl catalyst. I have tried thermogravimetric analysis, but I've got weight gain as the active Cu- get oxidised. Does anyone...

01 March 2021 1,907 3 View

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23 February 2021 4,014 3 View

Is ANSYS Fluent good for simulation of a three phase reactor(stusy of its hydrodynamics or mass transfer...)?

Hello everybody, I would like to simulate a three phase reactor and study its hydrodynamics. I would like to know which type of software is better? I have a good command of ANSYS Fluent and I am...

10 February 2021 4,303 3 View

Is there any books or journal that specifically discussed TPR in detail?

I am designing a metal oxide catalyst and among many characterization of this sample, TPR emerges quite significantly. The brief explanation in journal is limited to the sample that is...

21 January 2021 6,346 1 View

How cane capture of Cl, K , Na, in the process of biomass gasification, I want to let these elements enter the solid slag phase but not enter the gas?

I want to fixation Cl, K, Na in gasification slag, which type of catalyst can be used?

13 January 2021 2,145 2 View

How can I produce Alpha-monopropionin and Alpha-monobutyrin in lab scale?

I understand that glycerol should be reacted with propaoiocic acid to produce monopropionin and with butyric acid to produce monobutyrin , if you can help with the reaction conditions based on 1...

13 January 2021 1,151 4 View

How to calculate the concentration for as prepared nanomaterials?

if we are taking 2 materials of different concentrations (one is the precursor and the other one is the stabilizing agent) for synthesizing nanoparticles as a catalyst, how can I calculate the...

08 January 2021 516 4 View

What does this PZC data mean?

I measured different kinds of catalysts which were dispersed in DI water.I firstly added certain amount of HCl and then used NaOH to titrate and measure the pH value. What does this result mean?

27 December 2020 5,728 4 View

How can we choose the right method for Gaussian calculations?

I'm not working on biomolecules, I work on Organic, Organometallic, and sometimes inorganic structures. Do you know a simple way to apply the right calculation method? Is there any article or...

10 December 2020 3,396 5 View