I'm interested in how the secreted protein MMP-2 might be affecting a certain type of cell. I thought about adding the recombinant MMP-2 protein to in vitro cultures of the relevant cell type at different concentrations, but I can't seem to find any literature describing assays that do this so am not sure how best to approach it. Does anyone have experience of using metalloproteinases in this way?
Not sure I understand your question. Are you asking for possible outcome measures of an in vitro exposure to MMP2? if so, based on the type of cells you're culturing, you can look at tight junctions proteins (e.g. Zo-1 and claudin). These tight junction proteins are degraded by MMP9 and 2 (e.g. see Article Matrix Metalloproteinase-2 and -9 Secreted by Leukemic Cells...
).Also, the link below is a publication where the effects of MMP9 was evaluated in an in vitro model. It may give you some ideas on how to proceed. https://www.nature.com/articles/s41598-017-16250-3.pdf?origin=ppub
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