Sophie Quick

5 Questions 2 Answers 0 Followers

Questions related from Sophie Quick

Can I stain for neuronal spines in already cryosectioned tissue?

I have perfused and frozen rat brain tissue (from a new transgenic line) that I have cryosectioned onto slides in order to characterise a number of different cell types. One thing I would like to...

27 September 2021 1,655 1 View

What histological markers would show a stroke?

Defects in the brain tissue of a rat model I am working with look like small ischemic (not hemorrhagic) strokes when I do H&E stains. I would like to confirm this suspicion by staining the...

01 November 2019 8,719 2 View

A good anti Claudin 5 antibody for Fixed Frozen Rat brain tissue?

I'm interested in looking at the tight junctions of the blood vessels of the blood brain barrier in my rat model. I have perfused rats and cryosectioned the brains, but I am struggling to get a...

12 November 2018 5,564 2 View

How can I measure the effect of MMP-2 in vitro?

I'm interested in how the secreted protein MMP-2 might be affecting a certain type of cell. I thought about adding the recombinant MMP-2 protein to in vitro cultures of the relevant cell type at...

06 August 2018 1,009 1 View

Why has my human brain endothelial cell line failed to seed?

I recently bought in a new (very expensive) immortalised cell line, Human Brain Microvascular Endothelial Cells, or HBMECs. They were delivered in a vial on dry ice, I stored them overnight at...

26 June 2017 3,432 1 View