When preparing the figures for a paper, how should we write the name of the proteins and their phosphorylation state?

I think the rule is that the protein should be capitalized, but in the case of MAPK, people use the non-capitalized form (p38 and p42 for instance). Is that because it is a subunit? Related to...

10 November 2012 6,858 6 View

Why do most people think that melting of the arctic ice will not change the level of oceans?

I've heard so many times the argument of a melting iceberg floating in fresh water that will not change the volume of water when melting. But this is of course not valid for an iceberg floating in...

07 August 2012 1,484 7 View

Is it possible to extract RNA from 96-well plates for qPCR?

I need a method to measure gene expression by qPCR in very small samples, ideally cells grown in 96-well plates. What method should I use? Any suggestion will be welcome!

06 July 2012 6,732 8 View

How does one estimate the differentiation of THP-1 monocytes into macrophages?

Standard procedure to differentiate THP-1 cell is treatment with PMA and selection of adherent cells. Is there any specific markers that can be used to confirm the differentiation process, or that...

01 January 1970 704 10 View

What lipopolysaccharides should be used to mimic inflammation in obesity?

LPS can come from E. Coli, Salmonella and many other bacteria. Which one should be a good control to induce inflammation in animals and macrophages in culture?

01 January 1970 2,965 11 View

What connexins/pannexins are expressed in skeletal muscle?

There are several sub-types of pannexin and connexin channels that are expressed either during embryogenesis or in different tissues in adults. From your experience, does anybody knows which ones...

01 January 1970 2,674 5 View

How to get rid of proteins in supernatant from cells?

I want to remove/inactivate all proteins and peptides from my solution. I was thinking of using proteinase K to cut them all. Any idea of what concentration/time I should use, or any other method...

01 January 1970 5,963 6 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View