How can I measure nucleotides in culture media?

More Nicolas J Pillon's questions See All

When preparing the figures for a paper, how should we write the name of the proteins and their phosphorylation state?

I think the rule is that the protein should be capitalized, but in the case of MAPK, people use the non-capitalized form (p38 and p42 for instance). Is that because it is a subunit? Related to...

10 November 2012 6,937 6 View

Why do most people think that melting of the arctic ice will not change the level of oceans?

I've heard so many times the argument of a melting iceberg floating in fresh water that will not change the volume of water when melting. But this is of course not valid for an iceberg floating in...

07 August 2012 1,578 7 View

Is it possible to extract RNA from 96-well plates for qPCR?

I need a method to measure gene expression by qPCR in very small samples, ideally cells grown in 96-well plates. What method should I use? Any suggestion will be welcome!

06 July 2012 6,902 8 View

How does one estimate the differentiation of THP-1 monocytes into macrophages?

Standard procedure to differentiate THP-1 cell is treatment with PMA and selection of adherent cells. Is there any specific markers that can be used to confirm the differentiation process, or that...

01 January 1970 870 10 View

What lipopolysaccharides should be used to mimic inflammation in obesity?

LPS can come from E. Coli, Salmonella and many other bacteria. Which one should be a good control to induce inflammation in animals and macrophages in culture?

01 January 1970 3,063 11 View

How to get rid of proteins in supernatant from cells?

I want to remove/inactivate all proteins and peptides from my solution. I was thinking of using proteinase K to cut them all. Any idea of what concentration/time I should use, or any other method...

01 January 1970 6,051 6 View

What connexins/pannexins are expressed in skeletal muscle?

There are several sub-types of pannexin and connexin channels that are expressed either during embryogenesis or in different tissues in adults. From your experience, does anybody knows which ones...

01 January 1970 2,758 5 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Why is electronic energy 0.000 for liquid crystal compounds and is invariable with temperature in Guassian 09 software?

Are there any suggestions or insights you can provide to help address this problem

06 August 2024 1,443 2 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Silent Gene

See if this helps

http://www.perkinelmer.ca/fr-CA/CMSResources/Images/44-73201BRO_ATPlite.pdf

Ana Maria Oliveira Battastini

I would like also to know if with this "kit" we can measured ATP in the culture medium of cells without any treatment? The phenol red present in the culture medium does not interfere?