How can I measure multiple diameter's of a vessel within ImageJ?

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In need of Lambda max?

Can anyone assist in telling me the lambda max for the following compounds or a location to look them up? Brazilin Lawsone Vasicine Acetate Arjunic Acid Thanks

15 December 2023 7,736 0 View

How can I optimize my PCR reaction?

Hello, I have been trying to linearize a vector through PCR in prep for cloning for several weeks now. I am using Inverse PCR as opposed to enzyme restriction digestion. However, every time I have...

20 June 2023 6,689 1 View

I have found severely oxygen depleted iron particles in deeply buried alluvial sediments. Any ideas on what they could be and how they could form??

Any idea on what these may be and how they could have formed? They were found in deeply buried silty alluvial sediment samples from the Roanoke River in Virginia. Based on EDS measurements, the...

14 February 2023 3,507 3 View

Where can I find published methods for preparing composite diet samples for nutrient analysis?

I am working with a controlled feeding study, and we would like to homogenize all food on each daily menu and look at the content of a specific nutrient within the homogenized composite mixture....

07 February 2023 3,912 1 View

I want a simple protocol for estimating the concentration of intracellular amastigotes that does not require counting via microscopy?

Hi, So am challenged with the parasite rescue transformation assay of Leishmania. I lyse the infected macrophages for 30 secs with 0.05% SDS but the parasites are not rescued. I was able to...

13 November 2022 4,118 2 View

Can anyone help with my ZR-75-1 CRISPR K/O clonal expansion problem ?

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QPCR primer probe design tool for SNP detection?

Hello all, I am trying to find an online design tool like primer3 which will design primers and probes, but I need to be able to tell it where I want the probe to be. If, for example, I had a SNP...

23 March 2021 3,623 2 View

How to separate total protein into two fractions: large and small?

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16 November 2020 626 6 View

In Ansys HFSS, how do I replace all instances of a Local Variable within a Design with another variable?

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In Ansys HFSS, is there a method of creating an oscillating (AC) magnetic bias, without resonant peaks, within a non-magnetic substrate, e.g. Air?

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01 November 2020 6,032 3 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

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How Social Media Affects Your Mental Health ?

How Social Media Affects Your Mental Health

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Do you make articles from the Journal of Hypertension available (not the American Journal of Hypertension)?

I haven't seen one in a while so I wondered if that journal prohibits that form of redistriibution.

04 August 2024 5,137 3 View

PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View

How does social media influence people's dental habits and interest in aesthetic dental treatments?

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01 August 2024 9,349 2 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

Where to find a gene list for CRISPRa/i library screening of regulatory factors that affect pathogenic Th17 differentiation in PBMC?

I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...

31 July 2024 2,150 0 View

How to maintain the drug concentration in cell culture study for long term?

I am working in the filed of long acting drugs with Nano formulation. I want to study the efficacy of my formulation in 2D cell cultures while this required media change on every two days. In such...

24 July 2024 8,696 0 View

Can you visualize platelets using EVOS ?

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24 July 2024 9,337 3 View

Culture plates for PBMC (non-adherent). For infection?

Hello, good morning. I would like to know which plate I can use to infect fresh PBMC with virus and culture for 24 hours. I have tried using 12-well and 24-well plates, but many cells adhere and...

24 July 2024 2,158 2 View

Atilla Orhan

Hi. Maybe this link will be helpful. Good work. https://www.optinav.info/Local_Thickness.htm

Wei Wen Lim

Hi! Not sure if ImageJ has an equivalent plugin, but I find the 'Incremental Distance' tool on Image-Pro Premier very useful for measuring vessel wall thickness between two irregular shaped lines or polygons. Drawback though is that it's a paid software... a free version will definitely be useful to the general community. Good luck!

Video on its use on YouTube:

https://www.youtube.com/watch?v=clhLRF3tSbs