What is the half-life of TNF-alpha in vitro?

I'm designing a low-grade chronic treatment with this cytokine and I'm wondering how regularly to change the culture medium based on the half-life of the protein in an in vitro environment....

24 January 2021 5,988 2 View

I would like to know if there is any report of the average volume of the lung lining fluid in BALB/c mice ?

I would like to know the volume of lining fluid in mice

24 April 2019 8,716 3 View

Creating a new coordinate system in PETREL?

22 July 2018 7,099 14 View

HEK293 Cell line detachment after being adhered for over 3 days?

08 December 2016 9,523 2 View

Problems with the films for western blot developing?

Hi everyone! I am having some troubles when developing my western blot membranes using the film system: the films become really dark (even it is difficult to distinguish the bands) when I put them...

15 November 2016 891 7 View

Problems with the films for western blot developing?

Hi everyone! I am having some troubles when developing my western blot membranes using the film system: the films become really dark (even it is difficult to distinguish the bands) when I put them...

10 November 2016 7,734 8 View

What adherent substratum is appropriate for T cells, without any calcium current triggering?

24 October 2016 5,021 2 View

Best LC column for Plant Metabolomics?

Hi all!Typically we have been using a C18 Reverse Phase column for our studies, unfortunately there is no retention of most polar metabolites and all elute at very early retention times. This is,...

06 June 2016 8,133 10 View

How can I do a metabolite extraction for soil microorganisms?

Dear colleagues! I am trying to figure out how to do an extraction for soil microorganisms for further metabolomic analyses. I am only interested in the microbiota part, so I would like to discard...

08 December 2014 463 5 View

Mycoplasma elimination

We have developed several cell lines from human mammary tumors and they are contaminated with mycoplasma. We've tried BM Cyclin from Roche but they continue to be contaminated with mycoplasma...

14 April 2013 710 44 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Why is my platinum counter electrode getting dirty during electrodeposition?

I am conducting anodic electrodeposition of MnO2 onto a stainless steel substrate, using a platinum strip as a counter electrode. This is done galvanostatically, with the current held between 5...

27 February 2021 1,829 3 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

How do I choose contrasts in SPM8?

I am learning how to use SPM for PET and PET-MR analysis, but I haven't found any great resources that explain how contrasts work. I have a series of PET images where our hypothesis argues that...

24 February 2021 1,534 2 View

How to search for crystal structure for material with atomic position labelled?

I am using the crystallographic open database and materials project to search for cif file but the exact structure is not mentioned or how can I deduce that the given structure and atomic position...

23 February 2021 6,723 2 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

Can a semi-conductor material have different absorption coefficient?

Does the absorption coefficient of the semiconductor changes with the change in the structure of the semiconductor? If you have a thin film and nanowire of the same semiconductor, will the...

23 February 2021 9,799 4 View