Which is the preference between implementing sequencing or sending to sequencing?

More Mariella Rivas's questions See All

Which cell-penetrating peptides (CPPs) do you recommend to deliver intra-cellular cargo in chitin cell-walled unicellular eukaryotes?

Dear colleagues, I am working on a project in which I want to deliver oligonucleotides (specifically, molecular beacons (MBs)) inside different protist species. To do so, I am going to use...

18 March 2024 2,778 0 View

Which tool is the most reliable for predicting RNA secondary structure in oligonucleotides?

Dear colleagues, I am designing RNA molecular probes for FISH, and I want them to adopt a hairpin structure. I have checked for the possible secondary structure formation of some of the candidate...

14 February 2024 7,811 2 View

Respecto a familia ¿la presión que ejercen los padres sobre los hijos, podría repercutir en la ansiedad, tensión y presión arterial del adulto?

Cada vez estoy mas convencido de la relación de la salud mental con la salud física. La pregunta emerge, debido a dos preguntas previas: ¿porque algunas personas son muy ansiosas y se tensionan...

05 February 2024 6,441 0 View

There are non-invasive methodologies for glutamate measurement?

Measurement of glutamate by cerebral microdialysis, or by lumbar puncture, or otherwise by neuroimaging techniques such as magnetic resonance spectroscopy (MRS) and positron emission tomography...

04 November 2023 1,520 0 View

Wich technique to stain and make permanent samples of pollen is better for cactaceae?

Greetings! I´m on a proyect about cacti pollination. My team and I want to learn about pollen techniques to obtain permanent samples, as well with some stain procedure, once extracting it from...

26 June 2023 4,575 2 View

Which would be the most appropriate way to cite a photograph of our authority but it's uploaded to iNaturalist?

APA 7th ed.

03 May 2023 3,490 1 View

MIC determination and bacterial cell age, reason for it?

I know that "young cells" need to be used for MIC determination, I know that one of the reason for this is the presence of fat lipid bodies in some bacteria, are there other reasons reason for...

13 December 2021 2,297 3 View

What is the half-life of TNF-alpha in vitro?

I'm designing a low-grade chronic treatment with this cytokine and I'm wondering how regularly to change the culture medium based on the half-life of the protein in an in vitro environment....

24 January 2021 6,322 2 View

When administering the English version of a cognitive test which has been culturally adapted, is the original version or the back-translation used?

I am currently reading on the validation and cultural adaptation of the Montreal Cognitive Assessment (MoCA) in different countries and client populations. I wonder whether in the case of...

10 August 2020 7,418 3 View

Marginal effects or predicted probabilities in SPSS?

Is there a way of calculating marginal effects in the framework of a multinomial logistic regression in SPSS? Could someone help me? I can not find anything helpful about it. Thanks a lot, Mariella

12 June 2020 3,902 0 View

Which is the best model for hypoxia in cell culture ( MSC or fibroblasts)?

I am tring to culture cells in hypoxia using Cobalt chloride model , but I want to compare with antoher method. Our research is about circRNA expression - qPCR and nanopore oxford sequencing.

05 June 2024 2,246 1 View

Help on Modified Nanopore Sequencing to do Sanger-like sequencing for PCR amplicon from bisulfite converted DNA?

Hi, I'm planning to do sequencing using Nanopore. The main idea is to substitute Sanger Sequencing that usually used to sequence PCR amplicon from BCD (Bisulfite Converted DNA) using nanopore...

23 April 2024 6,637 0 View

How do researchers avoid over-amplification of eukaryotic chloroplast 16S rDNA?

How do researchers avoid over-amplification of eukaryotic chloroplast 16S rDNA when classifying prokaryotic microbial communities in algal bloom experiments? For example, if I need to amplify...

07 April 2024 3,064 0 View

Anyone help me to identify the species of the genus Berberis?

The photo of the species is attached for identification. We have sequenced the chloroplast genome of the species considering it Berberis julianae. However, now some discrepancies arise in the...

31 March 2024 7,917 1 View

ONT Nanopore (MinION): 16S rapid kit or Native kit for... 16S analysis?

Hey everyone, my question is maybe strange at first glance, but simple: is the rapid 16S kit's only real advantage the significantly larger 16S data amount generation? Shouldn't I be perfectly...

12 March 2024 3,909 2 View

What happen with my DNA extraction, why I keep having short fragment?

I'm doing DNA extraction from Streptococcus isolates for whole genome sequencing. I'm using DNeasy Blood & Tissue Kits from Qiagen, followed by 96 Rapid Barcoding Nanopore. The result keeps...

03 March 2024 6,322 1 View

What measures can be taken to improve NanoPore sequencing results, when working with a large amphibian genome despite meeting quality standards?

Greetings everyone. I seek guidance from individuals experienced in NanoPore sequencing of whole genomes of non-model eukaryotic organism. Our objective is to sequence the genome of an amphibian...

14 February 2024 6,075 5 View

How do I isolate chloroplasts devoid of nuclear contamination from non-model plant species?

Dear all, I've been trying for a long time to isolate pure chloroplasts from a few non-model plant species to obtain pure plastidial RNA. For example, I'm working with a fern (Adiantum capillus...

24 January 2024 5,838 0 View

Does anyone know the cause of plant protein gets brown and precipitates quickly how to get rid of those colors and precipitation problem?

We have expressed our protein in chloroplast when we add extraction buffer it get precipitate soon after centrifugation as this is real quick even we don't have a time to prepare sample for...

10 January 2024 9,727 10 View

RT-PCR with random priming giving >1kb cDNA?

Wondering if anyone is aware of a product and/or modified protocol to randomly amplify viral RNA to give long (>1kb) cDNA fragments. I use RT-PCR with random hex to give 300-500bp, which works...

04 January 2024 8,963 0 View

Carlos Bustamante

I haven´t tried the MinIon but I want to do it, please let me know how it goes or maybe I can help you to set it up in the lab. I found this quite interesting: Article MinION-Based DNA Barcoding of Preserved and Non-Invasively C...

Mariella Rivas

thanks Carlos, yes, I have been checking and they are supposed to have improved the error rate they had in the past, I found a paper in which they sequenced chloroplasts