Garshasb Rigi

5 Questions 1 Answers 0 Followers

Questions related from Garshasb Rigi

How can I make sure a protein with a signal-peptide is secreted or not?

There is a protein has two isoforms, one of which has a signal peptide. I am wondering if it is a secretory protein. If it is, how can I detect it? What if it is secreted by extracellular vesicles...

15 April 2019 2,831 0 View

Anti-Cancer Drug?

Is there any anti-cancer agent that no effect on cell survival but do effect other thing to suppress cancer proliferation/migration/invasion?

04 April 2019 6,800 3 View

How to remove endotoxin-LPS in protein?

Hi, I have one question. When express one protein (animal protein drug ) in E.coli system, E.coli endotoxin such as LPS will reside in the expressed protein. It is harmful to animal health. If I...

01 January 1970 432 2 View

What are the safety issues to consider in industrial recombinant protein production using E. coli?

I'm studying about recombinant protein production now. When producing recombinant protein in large scale using E. coli, there are several problems including endotoxin and bad smell etc. I want to...

01 January 1970 4,186 3 View

What is the best method for finding the binding partners of a secretory protein?

My protein is a secretory protein and I would like to find out what other proteins does it interact with (i.e. membrane proteins in the target cells). Ideally, I would like to screen for both...

01 January 1970 8,164 3 View