How can I make my own universal PCR mastermix then buying one that's already premade ?

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Bruno Salomone Gonzalez de Castejon

Yes feel free to make your own mastermix.

D.K. Kamalova

Based on the literature, you can create your mixtures and then optimise the protocol and proceed with the protocol that is optimal for you.

Krishna H Goyani

You can refer the below link for PCR MM composition:

https://www.promega.com/-/media/files/resources/protocols/product-information-sheets/g/pcr-master-mix-protocol.pdf?la=en#:~:text=PCR%20Master%20Mix%2C%202X%3A%2050,Information%20Label%20for%20storage%20recommendations.