Treatment of a large cross sectional unit in a panel setting?

I have a data set of 5 countries for 30 years and i want to analyse the relationship between y and x in panel setting. However, the countries are not similar in nature moreover. one country is...

30 January 2021 6,564 4 View

What is the solubility of Magnesium trifluoromethanesulfonate in methanol?

I am trying to dissolve Magnesium triflate in methanol. I took 2.6 g of it in about 20 mL methanol. After thorough stirring, I can see suspended particles! Can anybody throw some light on its...

12 January 2021 6,481 2 View

Could you explain the following situations, please?

I am trying to simulate a flexible antenna on ANSYS HFSS. Here, I have used "PDMS" as a substrate and "pec" as a probe (inner conductor) of a coaxial feeding. But during simulation, it is showing...

24 November 2020 800 5 View

Can I get an explanation for the following reason, please?

My plan is to fabricate a conformal microstrip patch antenna. I am going through the simulation works first. I already simulated the planar antenna. Now I am trying to simulate it with different...

19 November 2020 2,961 2 View

Anyone knows how to extract RNA from samples with high starch content (wheat) by using RNeasy Plant Minikit?

I am using 74904 to extract RNA from wheat seed and I have tried both of the lysis buffer. As it is mentioned it protocol, buffer RLT is the lysis buffer of choice but it may cause...

14 October 2020 9,409 7 View

What are the community-based primary prevention of NCD in LMIC country?

Please suggest types of literature to look for? Recommend journals which focuses on these issues.

12 October 2020 2,878 4 View

How write a concept note on Evaluation of community-based primary prevention models for NCDs?

1. What type of literature should I read for writing a successful concept note? 2. Is there any literature which will help me decide a research question/objective for designing a study will be...

12 October 2020 3,363 5 View

How long does it take for MCF-7 cancer cells to adhere to the microfluidic surface? Is 24 hours enough?

Hello everyone. We have cultured MCF-7 cancer cells in microfluidics several times and incubated them for 24 hours, but as the nanomaterial passed through the cells, the cells moved and no cell...

05 September 2020 7,292 3 View

Which software might be helpful to design and simulate the 3D shape of the planar/nonplanar microstrip/any antenna?

I am experienced with the antenna simulation using ANSYS HFSS and CST. But now I am working in an additive microsystems lab and want to move to 3D shape antenna simulation and printing.

03 August 2020 2,184 3 View

If I want to print the microstrip antenna using additive manufacturing techniques, do I need to design a 3D shape on CAD that I have created on HFSS?

I have designed and simulated a frequency reconfigurable on ANSYS HFSS, and now I want to print it using an inkjet-printing technology.

03 August 2020 7,439 4 View

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02 March 2021 3,060 3 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View