07 April 2018 1 9K Report

Hi dear researchers,

DMEM F12 glucose concentration is 17.5mM, I added D_glucose in basal cell culture until reached 30mM, but HUVEC cells had better grown than control group after 48 hours. I don´t understand this cause.

More Somayeh Sheikh's questions See All
What are the key differences between MMGBSA and MMPBSA in estimating free binding energy of a protein-ligand complex following Drug design study?

Please help to understand the aims and scopes of these two different techniques along with the possible computational cost that they render. Thanks.

25 April 2024 9,711 2 View

How much time does NCBI GenBank takes for public release of complete chloroplast genome sequence?

We have assembled a chloroplast genome of angiosperm and submitted the annotated assembly to NCBI GenBank. Within two-working days we got accession number for that submission. GenBank in their...

21 April 2024 9,974 0 View

Bioinformatics and Biology Insights of SAGE, does it have an impact factor?

I did not find IF in the website BioxBio for this journals but in their website they have claimed to have an IF of 5.8. Also did not find IF in the 2022 SCIE list (PDF) released by Web of...

12 April 2024 7,187 2 View

Top scientists in the field of Plant Molecular Systematics and Evolution? Could you please provide me a list?

So far I have found Prof. D. Soltis, Prof. John Cress and Prof. Jim Leebens-Mack. Please share your thinking. Thank you very much.

12 April 2024 5,028 1 View

Please suggest me some large databases of phytochemicals (plants/algae/fungi) for computer-aided drug design study?

Apart from IMPPAT, TIPDB, MeFSAT and Seaweed metabolite databases, could you please suggest me some more databases for virtual screening study. The target receptor is from...

09 April 2024 2,881 3 View

Have there been any articles about Fractal in Urban Planning since 2024?

"Have there been any articles about Fractal in Urban Planning since 2024?"

28 March 2024 7,165 0 View

I want to conduct a pilot study. What can be average number of questions that should be framed in a questionnaire?

The study is required as a pre-requisite for my research study at University.

21 March 2024 9,424 4 View

What novel techniques can be employed to detect and mitigate DDoS attacks targeting IoT devices?

Considering their resource-constrained nature and the need for real-time response.

19 March 2024 7,937 3 View

Is it mandatory to use a firewall if I employ an IDS or IPS?

1. Limited budget 2. the data being protected does not contain highly sensitive

17 March 2024 8,031 1 View

labeling method of selenium nanoparticles with radiopharmaceuticals?

I am working on biological selenium nanoparticles. I have a question related to labeling method of selenium nanoparticles with radiopharmaceuticals. How to achieve a yield above 90%??

17 March 2024 6,991 0 View

Similar questions and discussions
Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Are there any good simple systems or platforms to recommend?

In order to show people the beauty of control and enhance enthusiasm for learning control theories, are there any good simple systems or platforms to recommend?

05 August 2024 10,034 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close