Why the cleaved caspase 3 can't be detected in western blot?

I'm trying to detect the expression of cleaved caspase3 in mouse brain by western blot. Basically, my protocol is as followings: 1, lysis buffer: RIPA (ThermoFisher Science) 2, 50ug protein was...

07 August 2018 4,614 0 View

How to assess pAMPK/AMPK using western blot?

Hello, I'm trying to assess pAMPK/AMPK using western blot, which has been published in lots of literature. But I find the MW of both pAMPK and AMPK are 62KD. I think it is impossible to stain...

06 July 2018 3,736 0 View

Trouble in IHC staining?

I'm now trying to do double IHC staining on newborn rats brain sections. I have tried several times. It seems that it works well when the nucleus is to stained, such as BrdU/NeuN, but I always...

04 May 2018 3,972 5 View

Where to stand during the place trials of Morris water maze?

02 March 2018 9,232 8 View

Why the mice do not run into the escape box in the Barnes maze?

I am doing Barnes maze on AD and control mice these days. The protocol we use is as followings: 1, Put the animal on the maze and the escape box for 1 minute respectively to reduce the stress...

31 December 2017 8,113 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View