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I'm trying to detect the expression of cleaved caspase3 in mouse brain by western blot. Basically, my protocol is as followings: 1, lysis buffer: RIPA (ThermoFisher Science) 2, 50ug protein was...
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Hello, I'm trying to assess pAMPK/AMPK using western blot, which has been published in lots of literature. But I find the MW of both pAMPK and AMPK are 62KD. I think it is impossible to stain...
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I want to observe my cells proliferation in calcium free condition. I am culturing the cells in normal MEM media. We have bought a free calcium MEM medium, however, the new medium not only...
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I'm now trying to do double IHC staining on newborn rats brain sections. I have tried several times. It seems that it works well when the nucleus is to stained, such as BrdU/NeuN, but I always...
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We are doing MWM with mice. In my understanding, the experimenter should stand at the same location during all the trials of the place training， so that the mice will use the experimenter as a...
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I am doing Barnes maze on AD and control mice these days. The protocol we use is as followings: 1, Put the animal on the maze and the escape box for 1 minute respectively to reduce the stress...
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