Oil red O protocol for flow cytometry?

18 February 2021 4,214 3 View

After staining a gel with coomassie can I stain the same gel with fluorescent dye?

I want to know if it is possible to be done the inverse to view / verify other bands that not look in staining a gel with coomassie.

01 February 2021 8,647 5 View

What a 50% positive predictive value mean?

I want to explain a threshold of 50% PPV to describe how some assays perform when the prevalence of disease is high or low

07 October 2020 8,657 6 View

Author who is not actually an author of some of my publications?

I came out to realize that a person who is enlisted as an author of some publications that I am the REAL AUTHOR, is marked instead of me. How do I change that? Example: Standardized sampling...

21 September 2020 378 7 View

How to increase the short circuit current density in a perovskite solar cell?

I'm optimizing the procedure for making inverted PSCs: ITO / PTAA(8nm) / CsMAFA(320nm) / PCBM(60nm) / BCP(9nm) / Ag(100nm). The cells look very good, I have a very good FF (0.70) and Voc (1.0V),...

16 September 2020 6,286 8 View

How can I identify and catalog the Rainforest fungus?

We have a big area of Rainforest where we can see a big variety of fungos growing around. I'd like to catalog them using a simple methodology. We do not have equipment for that despite a...

16 March 2020 9,034 3 View

Sensors and Systems for IIoT?

13 March 2020 7,295 3 View

Does someone know the cause of stains appearing on n-i-p Perovskite Solar Sells?

I did the procedure described by saliba ( How to Make over 20% Efficient Perovskite Solar Cells in Reg... ) to make n-i-p PSCs. However, after perovskite deposition stains appeared in the cell...

29 October 2019 8,012 3 View

How to process small samples for histology?

Hi, I have very small samples of about 1x2mm (dental pulp tissue) and I need to process them for histology (dehydration, parafine inclusion, stainning with HE, etc). The problem is that the...

18 September 2019 1,180 4 View

What is the best protocol for evaluate TMR-peptide?

I apologize if my question seems to naive, but I would like to do a protocol to determine bio-distribution of a peptide coupled to TMR in an animal model however, I am having some difficulties in...

07 July 2019 5,664 2 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

How to quantify carbon deposition on CuZnAl catalyst?

Dear All, I want to quantify carbon deposition on the surface of the CuZnAl catalyst. I have tried thermogravimetric analysis, but I've got weight gain as the active Cu- get oxidised. Does anyone...

01 March 2021 1,907 3 View

Does any one can help me choose a reasonable basis set for intermediate metal ions interactions with guanine (i.e Fe2+, Mn2+, Cu2+ and exc.)?

Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...

01 March 2021 6,187 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What condition required for coating of Fe3O4@SiO2 by using APTES?

I tried coating Fe3O4@ SiO2 by using APTES in 5% water-ethanol but it is not coated. Kindly suggest a solution for it. Thanks

24 February 2021 5,992 4 View

Is the box plot of climatic indices better illustrate from daily data or annual data?

Hello, I hope you have a good time. I work on a research project about temperature indices. Due to the high number of indices, I only work on tables and maps on an annual time scale. In other...

19 February 2021 9,742 2 View

What is the best substrate for exfoliating few layers of hexagonal boron nitride (hBN) using traditional scotch tape method?

Unlike graphene, it is usually very difficult to exfoliate few layers (~10 nm thick) of hBN on SiO2 substrate due to lip-lip interaction at the edges. Au substrates have been proven very useful...

16 February 2021 2,635 2 View

How to avoid ATP degradation while performing ATP assay?

I am using the ATP Assay Kit (Colorimetric/Fluorometric) (ab83355) for measuring intracellular ATP from treated cell lines. Although my standards are wokring fine when using the fluorometric...

15 February 2021 3,746 4 View

Why do we need to neutralize the charge of the system by adding ions before we proceed to energy minimization step in GROMACS ?

I am learning MD simulations using GROMACS and while I was doing a protein in water MD simulation a doubt raised. I was following the steps mentioned on the tutorials page and it said The system...

15 February 2021 5,514 4 View

I want to remove TiO2 and SiO2 coating from surface. any one can help me?

I want to remove TiO2 and SiO2 coating from surface. I think that i should use HCl and MgCl2 and an oxidation agent such as potassium nitrate, but i dont know in which propertion should these...

14 February 2021 300 3 View