What could be exact way to remove invasive Prosopis juliflora from arid and semi-arid tropics of world where species is considered disaster ?

Prosopis juliflora is considered a noxious weed but its eradication is very costly inhivitive if no impossible ! We have very strong openion that it's spread could be chacked only if it's pods are...

25 January 2021 4,433 7 View

How does fluent FWH solve acoustic data at a location where no mesh is present?

In the simulation tutorial of the aeroacoustics around a cylinder by ANSYS, recievers are placed at a location where mesh isn't present, how does the solver predict the acoustics at that location...

16 October 2020 3,368 5 View

Problem with Cryosections?

Hi, I have a problem with cryosections while staining. Positively charged slides are used for cryosections, in spite of that during the staining process, the sections are washed away during the...

10 September 2020 3,767 3 View

Can we use 2, 6-dichlorophenol indophenol (DCPIP) method for analyzing crude oil degradation containing high sulfur content in the crude oil?

I was trying to find out whether my bacteria can degrade crude oil or not using DCPIP method that changes color after oxidizing hydrocarbons. But, I happen to order the crude oil containing 5%...

25 June 2020 9,801 2 View

What are the normal characterization of a nano fertilizer?

In order to design a nano fertilizer what should be the common tests?

31 May 2020 4,337 4 View

How can we decide the anode and cathode materials for half cell Li ion battery?

Also what can be the reasons for poor charge capacity followed by a high discharge capacity?

24 March 2020 7,046 6 View

Why my lenticrisprv2 plasmid after ligation of oligos is of smaller size than the expected?

after miniprep, I sequenced the plasmid. After sequencing I found that a region of plasmid was missing and its size if of around 8kb while it should be of around 12kb. It has happened to me for...

21 March 2020 736 3 View

HnE Protocol for the frozen section of cells encapsulated in hydrogels?

Hi all, I'm facing problems in staining the cryosection of the cells encapsulated in hydrogels. I'm using both charged slides as well as the albumin coated slides for the HnE staining. During the...

28 May 2019 1,197 5 View

VZV plaque assays?

Has anyone done plaque assays for VZV using toluidine stain?

10 March 2019 7,657 1 View

Trouble with protein ladder in western blot. what could be the reason?

I am using 4-20% biorad precast mini gel and protein ladder is also from biorad. I have attached the image of membrane. Running voltage- 100 V for 1 hr at room temperature and 70 V for 2.30 hr at...

18 December 2018 4,231 5 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

How to arrange coordinates and extract climate dataset out of MPI-ESM-MR netcdf files?

Have a nice day everyone, I'm stuck in extracting datasets out of MPI-ESM-MR netcdf files because the latitude ranges from -3*10^6 to 3*10^6 and longitude ranges -5,9*10^6 to 5,9*10^6. Plus, the...

28 February 2021 912 3 View