I am using other plasmids (e.g. C1/2/3/4) that may aid in interaction of the two proteins. However, the level of expression of plasmids (C1/2/3/4) is significant if I transfect same amount of plasmid. Hence I am unable to determine their effect on protein interaction. what should I do next? is that feasible if I make their expressions (C1/2/3/4) in the similar level? And how to make their expressions in the same level. Is there any paper that uses this method? Thank you very much
What about the use of bicistronic vector where two genes are expressed from the same vector?
If you are in eukaryotic cells - as suggested by the term "transfect" in your query - you have the option (as suggested by Dr. Ali) of a bi-cistronic vector, or alternatively using an IRES or a FMDV2A sequence depending on exactly what you are trying to do. In each case, more than one product can be driven off the same induction stimulus so the proteins may be induced stoicheometrically.
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