Mengya Wang

3 Questions 2 Answers 0 Followers

Questions related from Mengya Wang

How long can the cre mouse brains injected with AAV-DIO-ChR2-EYFP store at -80?

I have several cre mice injected with AAV-DIO-ChR2-EYFP. 4 weeks post-injection, I perfused them with PFA, put into sucrose and finally stored them at -80. I will look at the expression of EYFP at...

31 March 2020 5,283 0 View

How can I look at the interaction between two proteins?

I am using other plasmids (e.g. C1/2/3/4) that may aid in interaction of the two proteins. However, the level of expression of plasmids (C1/2/3/4) is significant if I transfect same amount of...

11 January 2016 9,621 2 View

How can I improve the transfection efficiency of DNA with bigger molecular weight (75kD)?

Hello~ I am trying to transfect GFP-N1-5-HT1a into N2a cells using lipofectamine 3000. Before, I tried GFP-C1-5-HT1a with the same experimental condition, and the transfection efficiency can be...

12 August 2015 6,069 6 View