How can I isolate the blood plasma without degradation, precipitation and/or loss of microRNAs of the blood?

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What is the best protocol for bone marrow cell culture? Is it critical to isolate the mesenchyme cells in advance?

I need to set a protocol to culture the bone marrow cell. I appreciate it if anyone help me.

22 June 2024 7,562 2 View

Whats the reason that CO2 absorption by nanofluids is more effective in continuous process, compared to the batch process?

I have studied and compared the enhancement of CO2 absorption by nanofluids in batch and continuous processes. i have found that the enhancement effect of nanofluids is more pronounced in...

04 February 2024 670 2 View

I've taken sulfide sediment but it passes through filter paper. How do I separate it from the solution?

Most of the elements I have in solution are Cobalt and Zinc. I have used Na2S to precipitate.

27 January 2024 934 0 View

The enhancement of carbon dioxide absorption by nanofluids is more in continuous process or in batch process?

17 December 2023 7,589 2 View

How can I draw a polarization diagram in microbial fuel cells?

Hello Can someone explain to me the steps for obtaining data to plot polarization in microbial fuel cells?

23 April 2023 2,931 1 View

How to calculate the shear modulus of intact rock numerically?

The shear modulus of intact rock is obtained from G=E/2(1+v). I want to numerically(FLAC3D or 3DEC) calculate the shear modulus of intact rock as the ratio of shear stress to shear strain. Has...

14 February 2023 8,637 1 View

Why does the microbial fuel cell under my review have a constant current with an external resistance of 10 to 1000 ohms?

Hello, I am working with a two-chamber microbial fuel cell with a carbon cloth electrode with a copper wire as a current collector and without continuous addition of anolyte. After the open...

11 January 2023 2,804 0 View

Why are the models generated by the prediction tools not correctly modelled according to their template structure?

To generate a model for my target protein I have been using a number of prediction tools. However, Phyre2 and I-TASSER servers do not provide me with the correct models. The sequence of my target...

29 September 2022 8,196 2 View

Can oxidation diffuse in effective cross section of sample during the hot compression tests in ambient atmosphere?

the sample is brass, the temperature is 855 C, the strain rate is 0.001 1/s

25 August 2022 703 0 View

Can I develop a questionnaire by combining two or more conceptual models?

I'm planning to develop a questionnaire (measurement model) for which I haven't found a comprehensive conceptual model. I was wondering if it's possible to combine two or more theories or...

01 August 2022 4,397 2 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View