MFC operation was conducted for 30 days to ensure that steady voltage is achieved for at least three consecutive cycles before output measurements. At this time, the biofilm at the anode is complete. You should also consider the most stable times in a feeding cycle. Because if it is too early, it is not the maximum time, but too late, the substrate is exhausted and does not give maximum capacity.
The current (I), unit: ampere (A) was calculated using Ohm's law, I = U/R, where U represents the voltage output, unit: volts (V) and R is the external load resistance, unit: Ohm (Ω). Power (P), unit: watts (W) was calculated by charging the voltage and amperage: P = U × I. The power density was calculated by the surface of the electrode; PD = P/Aanode, where Aanode is the total surface area of the anode electrode in square meters (m2).