Flavonoids are a group of plant secondary metabolites thought to offer health assistances through cell signaling pathways and antioxidant effects. These molecules are found in a variety of fruits, barks and vegetables. Flavonoids are polyphenolic molecules containing 15 carbon atoms and are soluble in water. How I can i isolate and separate polar flavonoids from methanolic extract easily.
Check https://www.intechopen.com/books/flavonoids-from-biosynthesis-to-human-health/isolation-and-structure-characterization-of-flavonoids
Also check https://www.sciencedirect.com/science/article/pii/S1878535214001816
Dear Abdur, this is a very interesting technical question which has been frequently discussed on RG. I also suggest that you directly search the "Publications" section of RG to find and access relevant publications on this topic. Fore example, please have a look at the following useful articles which have all been posted as public full texts on RG:
Isolation and Characterization of Novel Flavonoid from Methanolic Extract of Pongamia pinnata Pods
Article Isolation and Characterization of Novel Flavonoid from Metha...
Flavonoid from methanolic extract of Limoniastrum Feei (Girard) batt (Plumbaginaceae)
Article Flavonoid from methanolic extract of Limoniastrum Feei (Gira...
FLAVONOID FROM METHANOLIC EXTRACT OF LIMONIASTRUM FEEI (GIRARD) BATT (PLUMBAGINACEAE)
Article FLAVONOID FROM METHANOLIC EXTRACT OF LIMONIASTRUM FEEI (GIRA...
Isolation and Characterization of Flavonoid from Methanolic Extract of Mirabilis jalapa Linn. Tuber and Evaluation of its Cardioprotective Property
Article Isolation and Characterization of Flavonoid from Methanolic ...
Preliminary Phytochemical Screening and HPLC Analysis of Flavonoid From Methanolic Extract of Leaves of Annona squamosa
Article Preliminary Phytochemical Screening and HPLC Analysis of Fla...
and various others. Good luck with your research and best wishes!
Dear Abdur, P.S. You might also want to check the answers given to the following closely related technical questions which have been asked earlier on RG:
How to obtain non-polar, semi polar and polar fractions from a crude methanol extract?
https://www.researchgate.net/post/How_to_obtain_non-polar_semi_polar_and_polar_fractions_from_a_crude_methanol_extract2
(3 answers)
How to separate phenolic acids and flavonoids from methanolic extract of leaves?
https://www.researchgate.net/post/How_to_separate_phenolic_acids_and_flavonoids_from_methanolic_extract_of_leaves
(6 answers)
How to separate flavonoids from methanolic extract?
https://www.researchgate.net/post/How_to_separate_flavonoids_from_methanolic_extract
(5 answers)
and various others.
Silica gel chromatography is the main method to isolate or identify flavonoids. It is applied to isolate low or medium polar constituents. Reversed phase silica gel (e.g. reversed phase C18 silica gel) is commonly used to isolate flavonoid glycosides.
Here are some things to try:
Ion exchange, polyamide, and Sephadex LH-20 are good early clean-up steps.
https://www.teledyneisco.com/en-us/liquidChromatography/Chromatography%20Documents/Application%20Notes/Purification%20Strategies%20for%20Flavones%20and%20Related%20Compounds%20App%20Note.pdf
https://www.teledyneisco.com/en-us/liquidChromatography/Chromatography%20Documents/Posters/Purification%20of%20Phenolic%20Compounds%20Using%20a%20Combination%20of%20Flash%20and%20Preparative%20Chromatography.pdf
https://www.teledyneisco.com/en-us/liquidChromatography/Chromatography%20Documents/Posters/Purification%20of%20Phenolic%20Flavonoids%20with%20FC%20Poster.pdf
Please read the following book:
Isolation and Structure Characterization of Flavonoids
- August 2017
- DOI:
- 10.5772/67881
- In book: Flavonoids - From Biosynthesis to Human Health
- Publisher: InTech
- Editors: Dr. José Justino
- License
- CC BY 3.0
You could make a liquid-liquid extraction with immiscible solvents to eliminate the compounds of lower polarity.
Kindly visit
https://www.researchgate.net/deref/https%3A%2F%2Fwww.teledyneisco.com%2Fen-us%2FliquidChromatography%2FChromatography%2520Documents%2FPosters%2FPurification%2520of%2520Phenolic%2520Compounds%2520Using%2520a%2520Combination%2520of%2520Flash%2520and%2520Preparative%2520Chromatography.pdf
Kindly visit..
https://www.researchgate.net/deref/https%3A%2F%2Fwww.sciencedirect.com%2Fscience%2Farticle%2Fpii%2FS1878535214001816
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