I am interested in inducing chemical LTP in my model of ex-vivo slices from rat hippocampus. Does anyone know how can I do it? I men, which agonists of NMDa receptor can I use or which concentrations. Thank you!
Hi Marcel and thank you! Yes, I have read about modulation of the cAMP pathway and GABAa receptor inhibitors (could you give me the entire reference of the article by the way?) but I have read also about using glycine to activate the NMDA receptor. What do you think? a combine strategy would be excesive?
Ok, thank you very much for your point of view. I will continue looking into a bit more in order to make a decision
Please look at this review for various options/protocols: Molnár E (2011) Long-term potentiation in cultured hippocampal neurons. Semin Cell Dev Biol 22:506-513.
Could you recommend me any article regarding to the Gly-induced LTP in slices? I use slices and although your review is about cell cultures there is information about some protocols with slices, except for Gly protocol
A few years late, but...
Lucas, regarding your follow-up question on Gly-induced LTP in slices (rather than culture), see Article High concentrations of glycine induce long-lasting changes i...
Baudry also has some additional papers on glycine in acute hippocampal slices.
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