Why I couldn't obtain a gel from APTES? Does the Amine group affect the gelation process?

I am trying to make a gel from APTES as a precursor using the sol-gel reaction. I tried different solvents (ethanol, acetone, DMF) and different catalysts (basic and acidic) with different...

03 October 2020 9,673 5 View

How to estimate Lipid-Protein interactions durations from MD trajectories ?

Hi, I have trajectories of a protein embedded in an explicit membrane and water environment. I would like to estimate lipid-protein interaction durations i.e., the average duration of the...

26 October 2019 5,374 3 View

How can we separate between the fluorescent effect and second-order vibration in the wavenumber [1000-2000cm-1] in Raman Spectroscopy?

I am researching BaTiO3 ceramics and i find peaks in the wavenumber [1000-2000cm-1].How can we separate between the fluorescent effect and Second-Order vibration in the wavenumber [1000-2000cm-1]...

30 April 2019 9,125 8 View

How to read Gaussian Cube file?

Hi, I have a Gaussian cube file (.cube) that I obtained with gmx spatial. It represents the spatial density of cholesterol during a simulation (Protein in a membrane with explicit solvent). I...

21 April 2019 8,430 1 View

What is the difference between 633nm and 785nm lamp in Raman Spectroscopy?

I am researching rare earth doped pervoskite ceramics and found that most researchers used 633nm Laser. What is the difference between them and will there be much difference in the results?

15 April 2019 5,396 8 View

How to calculate the tilt angle of a protein (or a helix) with respect to the membrane plane?

Hi, I have a Coarse-grained simulation of a dimer with 5 transmembrane helices for each monomer and I would like to calculate the tilt angle of each monomer with the respect to the membrane...

24 January 2019 9,853 2 View

What is the lipid composition of the mitochondrial Outer Membrane (omm)?

Dear all, I would like to simulate a protein which is located in the Mitochondrial Outer Membrane (OMM). I was searching in the literature about lipid composition of this latter (in Mammals) but...

04 February 2018 5,094 2 View

Cyclophosphamide (Cy) and Scid mice !!

08 June 2017 536 7 View

Is it necessary to confirm the RNA-seq results by quantitative RT-PCR?

29 August 2016 1,740 3 View

How to generate a topology for a HEM protein using CHARMM 27 as FF?

Dear all, I'm working on simulating a homodimer with its cofactors (hem, THG) and its substrate with GROMACS. Since my system is complex, I decided to start first with a monomere taking only...

11 May 2016 6,272 2 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

How to control the variants vof covid 19 in second and third waves?

A the world find means to control covid 19 by vaccination ,new variants came to spread and make the world in fear and danger, to understand the conduct of these variants, es constantly change...

21 February 2021 3,080 6 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

How to calculate the TCID50 when the highest CPE is 50% or less?

I am doing TCID50 to measure progeny virus and some treatments protected the cells in a way that the highest CPE observed is 50% or less. How should I do the titre calculation in that case? Thank you!

16 February 2021 8,768 1 View

How to estimate/know plant virus concentration to make a standard curve in ELISA after serial dilution?

Hello, I am trying to know tomato virus titer/concentration in samples and I will perform serial dilution of the positive control. I wanted to ask How to estimate/know plant virus concentration to...

13 February 2021 6,534 4 View

What are the possible effects of COVID -19 Vaccine/s on the persons already recovered from the disease?

Persons recovered from COVID -19 are having antibodies against the virus inside their bodies. They are having T cells already sensitized to the antigens of the virus. If they are vaccinated (may...

12 February 2021 4,106 22 View