Hello,
I need to produce antibodies of swine influenza virus, I am trying to search for a protocol to inactivate the virus, would it be possible to provide a protocol? Thanks
Dear researchers, I am working on age classification of audio content, I need some labeled public datasets to train my model. Could anyone help? Thank you!
17 May 2021 7,558 4 View
Hi all, I am going to separate calcium chloride CaCl2–water mixtures. Given that, the concentration of CaCl2 is 7%. There any suitable method for separation and extraction of CaCl2?
26 April 2021 2,918 10 View
I am trying to make a gel from APTES as a precursor using the sol-gel reaction. I tried different solvents (ethanol, acetone, DMF) and different catalysts (basic and acidic) with different...
03 October 2020 9,934 5 View
Hi, I have trajectories of a protein embedded in an explicit membrane and water environment. I would like to estimate lipid-protein interaction durations i.e., the average duration of the...
26 October 2019 5,506 3 View
I have a DCP di calcium phosphate and phosphoric acid, and I want to synthesis the triple super phosphate (TSP), from this two reagents. How can i do please. Thinks a lot for your help
13 October 2019 7,130 1 View
In the temperature dependence of Raman scattering, why we use the Bose-Einstein population in Raman spectrum? and what is the purpose of this term?
26 June 2019 4,180 2 View
I am researching BaTiO3 ceramics and i find peaks in the wavenumber [1000-2000cm-1].How can we separate between the fluorescent effect and Second-Order vibration in the wavenumber [1000-2000cm-1]...
30 April 2019 9,462 8 View
Hi, I have a Gaussian cube file (.cube) that I obtained with gmx spatial. It represents the spatial density of cholesterol during a simulation (Protein in a membrane with explicit solvent). I...
21 April 2019 8,568 1 View
I am researching rare earth doped pervoskite ceramics and found that most researchers used 633nm Laser. What is the difference between them and will there be much difference in the results?
15 April 2019 5,646 8 View
Hi everyone, I'm working with immunosuppressed mouse model. Now I treated mice with cyclophosphamide (immunosuppressive drugs), so I need a protocol to measure the NK cell activity after...
30 January 2019 1,749 5 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
Pig slurry is rich in major and minor nutrients. Is there any way to improve / Enrich its manure quality to be used in agriculture organically ? please share your knowledge.
09 August 2024 5,605 2 View
Best method and number of embryos for extracting RNA from pig morulae?
21 July 2024 4,398 1 View
I am treating human cells with an AAV vector encoding for my gene of interest tagged by FLAG. I want to quantify the mRNA expression of my gene of interest via qPCR to discriminate the endogenous...
14 July 2024 8,482 3 View
I'd like to know if anyone has got experience in intracerebroventricular (ICV) AAV inj. I am looking for a concomitant spread of the AAV including but not limited to the motor cortex and the...
12 July 2024 781 0 View
I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...
11 July 2024 5,669 5 View
Hey everyone, I've been working with GRIN lenses (Inscopix) for almost 1 year and still had no luck seeing strong fluorescence on them. When I look at them, I can see good blood flow and,...
08 July 2024 5,178 2 View
I get two different bands from BCMV after PCR
07 July 2024 2,112 1 View
We have a couple of primers but when we make a blast for those primers we see two results: When I use Blast from NCBI, I found many lineages which anniling with many lineages. But when I want to...
01 July 2024 7,644 3 View
Pigging is basically a cleansing process followed in all pipeline industries after the product has been delivered. There are many types of pigs that are inserted inside the pipeline for this...
27 June 2024 1,221 1 View