Why I couldn't obtain a gel from APTES? Does the Amine group affect the gelation process?

I am trying to make a gel from APTES as a precursor using the sol-gel reaction. I tried different solvents (ethanol, acetone, DMF) and different catalysts (basic and acidic) with different...

03 October 2020 9,673 5 View

How to estimate Lipid-Protein interactions durations from MD trajectories ?

Hi, I have trajectories of a protein embedded in an explicit membrane and water environment. I would like to estimate lipid-protein interaction durations i.e., the average duration of the...

26 October 2019 5,374 3 View

How can we separate between the fluorescent effect and second-order vibration in the wavenumber [1000-2000cm-1] in Raman Spectroscopy?

I am researching BaTiO3 ceramics and i find peaks in the wavenumber [1000-2000cm-1].How can we separate between the fluorescent effect and Second-Order vibration in the wavenumber [1000-2000cm-1]...

30 April 2019 9,125 8 View

How to read Gaussian Cube file?

Hi, I have a Gaussian cube file (.cube) that I obtained with gmx spatial. It represents the spatial density of cholesterol during a simulation (Protein in a membrane with explicit solvent). I...

21 April 2019 8,430 1 View

What is the difference between 633nm and 785nm lamp in Raman Spectroscopy?

I am researching rare earth doped pervoskite ceramics and found that most researchers used 633nm Laser. What is the difference between them and will there be much difference in the results?

15 April 2019 5,396 8 View

How to calculate the tilt angle of a protein (or a helix) with respect to the membrane plane?

Hi, I have a Coarse-grained simulation of a dimer with 5 transmembrane helices for each monomer and I would like to calculate the tilt angle of each monomer with the respect to the membrane...

24 January 2019 9,853 2 View

What is the lipid composition of the mitochondrial Outer Membrane (omm)?

Dear all, I would like to simulate a protein which is located in the Mitochondrial Outer Membrane (OMM). I was searching in the literature about lipid composition of this latter (in Mammals) but...

04 February 2018 5,094 2 View

Cyclophosphamide (Cy) and Scid mice !!

08 June 2017 536 7 View

Is it necessary to confirm the RNA-seq results by quantitative RT-PCR?

29 August 2016 1,740 3 View

How to generate a topology for a HEM protein using CHARMM 27 as FF?

Dear all, I'm working on simulating a homodimer with its cofactors (hem, THG) and its substrate with GROMACS. Since my system is complex, I decided to start first with a monomere taking only...

11 May 2016 6,272 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Necessary tube lenght to cool Natural gas in a dry-ice cooling bath?

I have a tank which contains natural gas at 250 Bar and 293 Kelvin. I want to cool the gas in a dry-ice cooling bath to about 195 Kelvin. The gas will flow in a copper-tube through the bath with a...

27 February 2021 8,128 1 View

Exit velocity of natural gas from pressurized tank?

Hi Guys. I'm a mechanical engineer and have a bit trouble, would be very glad if you could help me with my questions. I have a pressurized tank which contains Natural Gas at 200 Bar and 293...

26 February 2021 6,253 3 View