16 Questions 20 Answers 0 Followers
Questions related from Zeineb Mhamdi
Hi everyone, I'm working with immunosuppressed mouse model. Now I treated mice with cyclophosphamide (immunosuppressive drugs), so I need a protocol to measure the NK cell activity after...
31 January 2019 1,782 5 View
Hello everyone, I am trying to produce immunosuppressed mice in my protocol so I want to understand the difference between scid mice and cyclophosphamide mechanism. Any suggested please?
09 June 2017 683 7 View
Hello, Im working with co-infection PRRS virus/Swine influenza virus, i'm waiting for RNA-seq data, so please I need to know Is it necessary to confirm the RNA-seq results by quantitative RT-PCR...
30 August 2016 1,890 3 View
Hello,I am just do co-infection between swine influenza virus and porcine reproductive and respiratory syndrome virus (PRRSV). I will test differents conditions and I need to detect genetic...
01 April 2016 633 3 View
HI I’m working with swine influenza virus, by sequencing I have detected some mutations on M2 and N genes, so I need tested resistance from these genes against antiviral drugs (oseltamivir and...
01 December 2015 2,735 3 View
Please I need to inactivate some influenza viruses by UV, any suggestion about protocol please Thank you
11 June 2015 7,135 4 View
Hello I have two questions 1- I need to understand difference between complete and incomplete adjuvant 2- For Influenza viruses for example when we immune same pigs with inactivated viruses to...
26 May 2015 8,307 3 View
Hello, Actually I deactivate influenza virus (swine) with formaldehyde, but by infectivity test on MDCK cells I see cytopathic effects on the first line, so I re-inactivated for the second time...
25 May 2015 265 4 View
Hello 1- Im interstand to virus-like particle vaccines, on Porcine Reproductive and Respiratory Syndrome (PRRS), I need to understand her concept, and can you suggest me some...
14 April 2015 7,582 6 View
Hello Please I nead same help about interpret results of Mixed effect logistic regression model, FOR EXPVaccination: XvStatus vaccine (v=vaccination) : OR= 0.6, SO vaccination its...
14 April 2015 6,433 4 View
I am currently in the process of purifying influenza virus on a sucrose gradient but still I have low amounts of protein, you can give me suggestions if not, I use OptiPrep™ Density Gradient...
16 March 2015 9,636 9 View
Hello 1- Q: I work on the swine influenza virus, I want to know if the results of the genetic characterization of the virus are sufficient to get an idea on the mode of evolution, or is antigenic...
28 January 2015 8,963 3 View
Hello I am trying to find a method to inactivate swine influenza viruses that are produced in eggs, most of the items gives formalin, but I find a specific protocol, I need suggestions and ideas...
28 November 2014 4,652 16 View
Hello, I need to produce antibodies of swine influenza virus, I am trying to search for a protocol to inactivate the virus, would it be possible to provide a protocol? Thanks
21 November 2014 9,311 0 View
hello I work on the genetic and antigenic variability of influenza virus in swine. Is the observed variability be due to PCR or are they all real? Why do my MDCK cells and eggs are mainly used to...
09 September 2014 6,835 4 View
Hello there I work on a project influenza, and I am trying to isolate virus from lung tissue of pigs but unfortunately on 8 samples I can not see a cytopathic effect is what you can give me...
24 August 2014 8,705 3 View
