I am performing whole genome sequence for gram negative acinetobacter baumannii using two platforms illumina miswq and oxford nanopore. Thus i require a very high yield and high purity.
I am using Zymoresearch DNA kit for extraction and DNA/RNAse free water to elute.
Please can you suggest how can i improve my purity that is giving high ratio for 260/230 for e.g 4.07
A low 260/230 ratio generally means high salt contamination and in particular guanidium salts that are present in Lysis buffer to protect your nucleic acid from nucleases
To increase your 260/230 ratio you add your ethanol based wash buffer to column; wait 1 min; spin then repeat this step
If you cannot re purify you either need to precipitate your material and wash the pellet x2 with room temp 70% ethanol or put down another column and do the above
Guanidium salt contamination of your purified material associated with 260/230 ratio < 1.0 and especially
Tasnuva Avzun You may follow this thread for more answers https://www.researchgate.net/post/How_to_improve_my_260_230_ratio_for_DNA_high_purity
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