Hi All I am planing an experiment to re-sensitize the colistin resistant gene in Klebsiella pneumoniae. For this i have two plasmid system one carrying the Cas9 and lambda red recombination and other carrying the gRNA with SSoligonucleotide i.e the repair template for editing the insertion sequence in the chromosome.My concern is how can i use this two plasmid to package in a phagemid for efficient delivery in the target cell.