If a miRNA structure is modified by addition of a methyl Fluoro group or LNA, does this affect the PCR efficiency of the TaqMan real time probes?

11 November 2016 1 2K Report

We have modified the miRNA structure in different ways as mentioned in the question at multiple nucleotides of the mature MiRNA strand duplex mimics. Is there a chance the SYBR primers may be a better choice to test over-expression in this scenario.

Tasnuva Kabir

Hi Sheik. Thank you for the reply. Have you done something similar?

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