I am ordering cryopreserved PBMCs. I want to thaw these cells to inject into NSG mice. Best way to thaw/rest them prior to injection?

I have seen a variety of methods that people use to rest after thawing PBMCs to inject them into mice including: (1) Thaw, wash in HBSS, and inject right away (no resting) (2) Thaw, and then...

16 October 2019 5,133 2 View

Can DMSO increase cell viability in MDA-MB 231 breast cancer cell?

I am currently doing MTT assay on MDA-MB 231s and I treat the cells with 0.2% of DMSO (vehicle control). the problem is that the DMSO seems to increase cell viability (above 100% as compared to...

08 June 2019 5,884 2 View

Which coding program do you recommend for analysis of electrophysiological recordings, and why?

I need to do analysis of spike frequency, AP shape, AHP, epsps, and compare those across different cell types and conditions. I usually use clampfit, and have a basic knowledge of Igor. But I...

14 March 2016 6,657 5 View

Convert dataset to tensors

How to convert a dataset which has two items-image and label , where image is depicted with a list of image names such as '12_left',12_right' and so on, and labels such as 0,1,2,3,4? Tensorflow...

01 January 1970 9,731 6 View

Conversion of images to arrays

How to convert image dataset in device (not the inbuilt dataset as found in tensorflow or sklearn) into a numpy array?

01 January 1970 4,188 7 View

How to perform cross validation on larger image dataset using Convolutional Neural Networks and Deep Learning?

Cross validation on larger image dataset is possible in machine learning through K-fold and various other Cross validation algorithms. But in case of Deep Learning, the same process cannot be...

01 January 1970 351 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View