I have GWAS data from Ilumina platform (The HumanOmni2.5-8 (Omni2.5) BeadChip). The data is in Plink format. When i tried imputation with IMPUTE2 i got a concordance rate of around 50 percent, which clearly states that I have some issues in data. I guess it could be a strand flipping issue or NCBI genome build. I am aware of 'flip strand' and 'scan for strand issue' options in Plink. The scan option in Plink required the phenotypes: cases and controls. But in my case all subjects are single batch and there were no cases and controls. Second thing is NCBI genome build. IMPUTE2 require the data to be of NCBI bulid 37. I am aware of liftover tool. But again I should know what build is my data to do that. So I have two questions:

  • How do i diagnose if i have a strand problem in my GWAS data?
  • How do i make sure if i have all SNPs are annotated in forward strand?
  • How do i know what NCBI build is my data ?
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