Just extract the secondary metabolite from the broth by using any of the suitable solvent. And check the bioactivity to confirm.

see the link

https://www.researchgate.net/post/How_antimicrobial_compound_can_be_extracted_from_bacterial_culture

Thank you Vijay sir

can you send any reference articles or protocol

Check this related thread for answers to your question:

https://www.researchgate.net/post/Is_a_GC-MS_of_crude_extract_feasible/1

http://www.slideshare.net/rahulbs89/extraction-of-plant-contituents

suggested reading is

Antimicrobial screening and active compound isolation from marine bacterium NJ6-3-1 associated with the sponge Hymeniacidon perleve

Thank sir

Check these related threads for more posts:

https://www.researchgate.net/post/How_to_estimate_quantitatively_secondary_metabolites_in_plants#55505b625cd9e33d0a8b458a

https://www.researchgate.net/post/which_parameter_and_method_should_follow_for_Identifiaction_purifacation_and_characterization_of_crude_extract?_tpcectx=post_detail_recommendations&tpr_view=c4d19b8e-afee-4c52-b0ea-716e4e1aa61e_3&_trid=9d281620-8bb6-4a97-b162-858044542098_3

https://www.researchgate.net/post/Can_someone_list_out_the_methods_of_extraction_crude_extract_compounds_isolation_purification_identification_and_structural_elucidation

https://www.researchgate.net/post/How_we_can_identified_the_compounds_present_in_plant_extracts_without_using_chromatographic_and_spectroscopic_methods

https://www.researchgate.net/post/What_is_the_difference_in_nature_of_phytochemical_compounds_present_in_hexane_fraction_obtained_from_the_following_method

Thanks Dear Shaimaa for giving valuable information. How to precipitate before going analysis? can you give some suggestion?

doi.org/10.1007/s13596-019-00410-z

it depends that what your aim is?

are you going to identify a spacial metabolite? if yes...you can search and find its kind