I am currently working on a project that requires hormonal assay for the above mentioned hormones in west african dwarf bucks using human serum EIA Kits obtained from Inteco diagnostics UK Ltd
Article Development of a Simple Enzyme Immunoassay for the Determina...
Compare serial dilutions of goat serum with two standard different curves, one using purified human LH, FSH and prolactin and one using goat LH, FSH and prolactin (if available). If the goat standards are not available, test parallelism between the standard curve and the dilutions of goat serum. If the slopes in the linear portions of the curve are parallel, then you can use the human standards. If not, you may need to develop an adjustment factor to correct for non-parallelism. Make sure your samples fall in the linear portion of the curve. If they are not, increase or decrease the volume to make sure the values fall within the linear portion. Testosterone is the same in both species, so it should work either way.
For testosterone, there is no problem, so you can use human kits as such. For LH, FSH and Prolactin potentially there could be a problem because of the cross-reactivity as explained by Jack Britt and it may not work. EIA kits are available certainly for the ovine LH, FSH and possibly for prolactin as well which could be used for goat serum without any problem.
We run assays (RIA) for bovine, ovine and equine LH and FSH, also for bovine PRL. I imagine that they should work with goat serum. We could run some assays for you to cross check.
Alternatively the parallelism check suggested by Jack Britt need to be checked to allow you determine if the assay you have is valid for goat serum. If it is not parallel this suggests problems in serum interfering or problems with lack of 100% cross reactivity between the antibody detecting the human protein hormone and goat. The testosterone assay should work across species as it is a steroid hormone that is the same across species, but still some basic validation needs to be carried out to check that there is nothing in goat serum that interferes with the assay.
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