I know a few different methods to specifically label RNAs with fluorophores. I was wondering if I can also do this enzymatically within a bacterial system. This means I would like to know whether there is an enzyme which adds a certain fluorophore to RNA sequences (preferably to a sequence of my choice)? I am open for all kinds of information on this topic, but the labeling should happen within a bacterial or lower eucaryotic system (e.g. yeast).
Thank you for the ideas.
Not sure whether you can download but please follow the following link:
http://cmgm.stanford.edu/herschlag/publications%20%28PDF%29/SolomatinMIE_469p47.pdf
Hej Yang-Kao,
thank you for the review. I have read it unfortunately already :) It didn't 100% match what I am looking for but thank you nevertheless.
Hej Anna,
thank you for the thought. I was thinking about exploiting CRISPR already and it is nice to know that you are with me on this idea. We might develop this into an iGEM project here in Stockholm, but gonna see how far we get. If anyone is interested to follow-up on our iGEM project, follow us on FB for instance: https://www.facebook.com/STHLMIGEMCLUB
Thank you both again for sharing. And for all the others, please follow annas example and think aloud! :)
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