How to extract protein from adipose tissue for Western Blot?

Hi RG community, I want to perform Western Blot on murine adipose tissue. I am currently struggling with some issues to get good protein samples. I usually have been isolating adipose tissue...

06 July 2018 5,302 2 View

Are lymph nodes commonly removed from fat tissue before analysis of immune populations in fat tissues?

I am interested to know if people studying adipose tissue resident immune cells normally remove all lymph nodes or if that is not always possible and therefore people leave them in the fat and...

04 May 2018 9,541 1 View

How can I increase bacterial outer membrane permeability without killing the cell?

Dear research community,I have met an interesting issue in a small research side project in which I am engineering some bacteria. For this though I would need to permeabilize the outer membrane of...

10 November 2015 9,860 10 View

Do ligand-gated ion channels (which can be activated from the cytosol) exist in bacteria? What is their name? In which organism?

For a project within the iGEM competition, we are willing to construct a new biological biomarker detection system. For a read-out, we are considering an electric readout. Therefore, we want to...

04 May 2015 1,166 9 View

Can Bacteria (e.g. E.Coli) take up small non-coding RNA from their environment?

For a small play of thoughts. I was wondering if bacteria, despite their complexe cell membrane-cell wall structures is capable of taking up miRNAs from its environment. Even if it doesn't have...

02 March 2015 6,808 0 View

How can I fluorescently-label RNAs using an enzymatic reaction?

02 March 2015 1,544 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

Where can I find atmospheric measurement profiles (temperature, density, etc.) between 300-800 km altitude?

I am investigating thermospheric mass density profiles between 300-800 km altitude. I would like to compare the variability with other parameters, including temperature, electron content,...

01 March 2021 5,902 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View