How to extract protein from adipose tissue for Western Blot?

Hi RG community, I want to perform Western Blot on murine adipose tissue. I am currently struggling with some issues to get good protein samples. I usually have been isolating adipose tissue...

06 July 2018 5,302 2 View

How can I determine HDL in bone marrow?

I would be interested in the levels of bone marrow HDL and LDL. I was wondering if I could use kits that are designed for serum? Or if I can alternatively use ApoA1 or ApoB in Western Blot...

08 September 2017 3,652 4 View

How can I increase bacterial outer membrane permeability without killing the cell?

Dear research community,I have met an interesting issue in a small research side project in which I am engineering some bacteria. For this though I would need to permeabilize the outer membrane of...

10 November 2015 9,860 10 View

Do ligand-gated ion channels (which can be activated from the cytosol) exist in bacteria? What is their name? In which organism?

For a project within the iGEM competition, we are willing to construct a new biological biomarker detection system. For a read-out, we are considering an electric readout. Therefore, we want to...

04 May 2015 1,166 9 View

Can Bacteria (e.g. E.Coli) take up small non-coding RNA from their environment?

For a small play of thoughts. I was wondering if bacteria, despite their complexe cell membrane-cell wall structures is capable of taking up miRNAs from its environment. Even if it doesn't have...

02 March 2015 6,808 0 View

How can I fluorescently-label RNAs using an enzymatic reaction?

02 March 2015 1,544 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View