How to extract protein from adipose tissue for Western Blot?

Hi RG community, I want to perform Western Blot on murine adipose tissue. I am currently struggling with some issues to get good protein samples. I usually have been isolating adipose tissue...

06 July 2018 5,302 2 View

Are lymph nodes commonly removed from fat tissue before analysis of immune populations in fat tissues?

I am interested to know if people studying adipose tissue resident immune cells normally remove all lymph nodes or if that is not always possible and therefore people leave them in the fat and...

04 May 2018 9,541 1 View

How can I determine HDL in bone marrow?

I would be interested in the levels of bone marrow HDL and LDL. I was wondering if I could use kits that are designed for serum? Or if I can alternatively use ApoA1 or ApoB in Western Blot...

08 September 2017 3,652 4 View

How can I increase bacterial outer membrane permeability without killing the cell?

Dear research community,I have met an interesting issue in a small research side project in which I am engineering some bacteria. For this though I would need to permeabilize the outer membrane of...

10 November 2015 9,860 10 View

Do ligand-gated ion channels (which can be activated from the cytosol) exist in bacteria? What is their name? In which organism?

For a project within the iGEM competition, we are willing to construct a new biological biomarker detection system. For a read-out, we are considering an electric readout. Therefore, we want to...

04 May 2015 1,166 9 View

How can I fluorescently-label RNAs using an enzymatic reaction?

02 March 2015 1,544 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

How can we simulate an hyperelastic-viscoelastic behavor with Ansys APDL?

I am working on modeling and simulation of biomecanical material behaviour, I have succeded on simulating skin using anisotropic hyperelastic material, on APDL and in our lab's finite element...

28 February 2021 552 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Statistics: include groups of respondents made by one person for analysis?

Good morning, I could not find literature about this so I am asking. I am organizing data for a questionnaire (N=100) that foresees some personal questions like level of education. For the aim...

25 February 2021 2,090 4 View

Modeling viscosity using model of Chung?

Hello, As part of simulation of gases mixture and water, I need to calculate viscosity of the fluid components and I am using relationships proposed by Chung et al. (1988). The irony is while I...

25 February 2021 8,053 5 View