How can I find the DNA sequence of the Intracellular domain of NOTCH 1 receptor??

16 May 2023 1 5K Report

To induce Notch signalling in Huh 7 cells, I plan on cloning the NICD and then using it to activate the Notch Pathway. I need the region in the DNA that specifically codes for the Notch 1 intracellular DNA.

Subbroto Kumar Saha

Hi Umang

We got the Notch1 intracellular domain (NICD) as a gift from another university.

However, You can find the sequence below link-

https://www.addgene.org/91897/

Hope, this will help you. You can buy it from Addgene ($85).

Best wishes,

Subbroto

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Science method

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