To induce Notch signalling in Huh 7 cells, I plan on cloning the NICD and then using it to activate the Notch Pathway. I need the region in the DNA that specifically codes for the Notch 1 intracellular DNA.
Hi Umang
We got the Notch1 intracellular domain (NICD) as a gift from another university.
However, You can find the sequence below link-
https://www.addgene.org/91897/
Hope, this will help you. You can buy it from Addgene ($85).
Best wishes,
Subbroto
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