Umang Tyagi

3 Questions 1 Answers 0 Followers

Questions related from Umang Tyagi

How can I find the DNA sequence of the Intracellular domain of NOTCH 1 receptor??

To induce Notch signalling in Huh 7 cells, I plan on cloning the NICD and then using it to activate the Notch Pathway. I need the region in the DNA that specifically codes for the Notch 1...

16 May 2023 5,189 1 View

While plating Huh7 cells in a 12 well plate, cells are often concentrated in the centre as opposed to an even distribution. What could be the reason?

0.1 million cells in 1 ml media were added per well. Same pattern observed in all 12 wells during multiple platings.

16 May 2023 4,943 3 View

Does notch signalling pathway exist in yeast?

SInce Notch is a cell signalling pathway, and yeast is unicellular, does notch exist in yeast?

02 October 2022 3,205 3 View