I have osteoarthritis patient DNA. Now I want to study the interaction of the DNA with Vitamin B complex. What is the procedure to do so? What types of materials and methods are used to study this interaction? Is there any specific Protocol for study this interaction? Is there any paper on this topic?
Please tall me.
Thank you.
Why are you looking for interaction of DNA in osteoarthritic patients with vitamin B complex ? Do you have any hypothesis or idea ? I do not think you will find any relationship in the pertinent literature. The properties and functions of B vitamins are related to variable skin, neurologic and psychic disorders, and not to such affecting the musculo-skeletal system. A study on the suggested interaction seems to me to be of a "pure science" character.
There are some good studies than can give you ideas about where to look, I would start looking the combinations with Diclofenac the possible synergistic effects of Vitamin B complex an are not clear but the possible answer could be in some of these mechanism: increase in the availability of substances that Blockade nociceptive transmitters, also it have been reported a opioid mechanism and nitric oxide liberation and this have been probed to some degree with the inhibition of the analgesic effect of vitamin B complex trough the administration of naloxone an L-NAME.
Here are some works that will give you a good idea about where to look including one in osteoarthritis patients.
1. Magaña-Villa M, Rocha-González H, Fernández del Valle-Laisequilla C, Granados-Soto V, Rodríguez-Silverio J, Flores-Murrieta F, et al. B-vitamin Mixture Improves the Analgesic Effect of Diclofenac in Patients with Osteoarthritis: A Double Blind Study. Drug Research. 2013 Mar 22;63(06):289–92.
2. Dehghan M. Comparative Effectiveness of B and E Vitamins with Diclofenac in Reducing Pain Due to Osteoarthritis of the Knee. Medical Archives. 2015;69(2):103.
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