How can I extract the liquid (body fluid) sample from the PVA sponge?

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Isam Eldin Hussein Elgailani

Please look at the following below links which may help you in your analysis:

https://patentimages.storage.googleapis.com/bb/bd/6e/6ee568fd6fdd4e/GB2505557A.pdf

https://www.kinderkrebsinfo.de/patients/pohkinderkrebsinfountersuchungen/biopsies_and_punctures/index_eng.html

Thanks

John Carter

Can you centrifuge them?

Busra Kose

John Carter Yes, I will centrifuge them. But this PVA material retain the content of liquid sample very closely. So, I must keep wait in the buffer and shake in the buffer to extract them, to recover them very well.

Isam Eldin Hussein Elgailani Thank you for your recommendation, but these protocols not related to my experiment unfortunately.

You could put the sponge in a plastic bag with ELISA buffer (PBST?) and squeeze it several times.