How can I extract Jasmonic acid from plant leaves for High Performance Thin Layer Chromatography (HPTLC) isolation and quantification?

More Benji Brayan Ilagan Silva's questions See All

Where can I find the standard absorption spectrum of Jasmonic acid?

I need to compare the absorption spectrum of the compound I have isolated. I am suspecting that it is JA. I need a literature or a published result on the absorption spectrum of JA.

03 April 2016 7,499 1 View

What is the best method to visualize Trichoderma hyphae in the cortical region of the young roots of a tree?

I have tried aniline blue and cotton blue staining for root sections. They're not effective, in this case.

01 February 2016 3,287 4 View

Is there a protocol for simultaneous quantitation of jasmonic acid and salicylic acid using HPTLC?

I would like to measure the salicylic acid and jasmonic acid production of my subject plants as their response to insect pest. I would like to conserve the use of their leaves so as to minimize...

09 October 2015 6,757 2 View

How can I quantify salicylic acid from plant leaves using spectrophotometry?

I am trying to establish the effect of priming (using a fungus) to a plant.I want to determine the level of and changes in the amount of salicylic acid as time progresses. I was not allowed to use...

09 October 2015 4,958 3 View

Have there been published studies on the use of Trichoderma-induced systemic resistance against insect pests?

I'm currently starting my studies on the use of Trichoderma-induced systemic resistance against a newly identified insect pest. I want to know if there have been studies that also deal with ISR...

03 April 2015 4,182 1 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

The Bigger You Are, the Harder You Fall (some lessons from Dinosaurs)?

Evolutionary fitness is based on an organism’s ability to adapt rapidly to changing environmental circumstances. Large-bodied mammals have been equipped with large brains (and hence a high...

06 August 2024 4,849 2 View

Neeraj K. Sethiya

May these links help you.

http://www.ncbi.nlm.nih.gov/pubmed/18618474

http://www.sciencedirect.com/science/article/pii/S0003269702004669

Gunawan Indrayanto

Hi, in addition please search in this CBS Camag Data base, there are 2 publications more regarding HPTLC of jasmonic acid., best regards

http://www.camag.com/en/tlc_hptlc/customer_magazine_cbs/ccbs_database.cfm?method=query

Asit Ray

you can check out this website

http://www.ncbi.nlm.nih.gov/pubmed/18402723