I have tried aniline blue and cotton blue staining for root sections. They're not effective, in this case.
You can try: 1) methylene blue to distinquish dead and alive cells of fungus and root.
or 2) Gram staining procedure too see the dark blue fungal cells.
I use the GFP inserted in trichoderma and it clearly appear when you see under the fluorescence microscope
I think you can try Ink and Vinegar.
Vierheilig, et al. "Ink and vinegar, a simple staining technique for arbuscular-ycorrhizal fungi." Applied and environmental microbiology 64.12 (1998): 5004-5007.
wheat germ agglutinin (WGA) staining
I need to compare the absorption spectrum of the compound I have isolated. I am suspecting that it is JA. I need a literature or a published result on the absorption spectrum of JA.
03 April 2016 7,434 1 View
I would like to measure the salicylic acid and jasmonic acid production of my subject plants as their response to insect pest. I would like to conserve the use of their leaves so as to minimize...
09 October 2015 6,696 2 View
I am doing a research on the effect of priming to the levels of jasmonic acid produced by a plant. I will be using the leaves, and to quantify it, I will beusing HPTLC.
09 October 2015 8,351 3 View
09 October 2015 4,852 3 View
I'm currently starting my studies on the use of Trichoderma-induced systemic resistance against a newly identified insect pest. I want to know if there have been studies that also deal with ISR...
03 April 2015 4,126 1 View
02 March 2021 3,060 3 View
What do you know about biotechnology art?
02 March 2021 1,653 3 View
I am searching for a good place for the Post Doc,
02 March 2021 4,053 3 View
I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...
01 March 2021 7,675 3 View
Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...
01 March 2021 1,133 1 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...
28 February 2021 7,356 8 View
The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.
28 February 2021 9,464 2 View
Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...
28 February 2021 9,138 2 View
Or will we have to get vaccinated every year, as in the case of influenza?
27 February 2021 1,048 5 View