Hi Mohsen,

I can suggest an efficient method describe by  Della Porta et al., 1983  Plant Mol. Biol. Rep. 1, pp. 19-21 for plant DNA miniprep but useful also for nematodes.

Regards

 Many Thanks from thousands far.

Dear All

I am doing  PCR on a COX II gene on a nematode using the followings primers 

COXII F TCT AGT TAT ATA GAT TGR TTT YAT

COXII R CAC CAA CTC TTA AAA TTA TC

It is mention to cite I have to use this primers to get a 629 bp Band the problem

that I face back to  the  primers as my PCR just work well for less than 10 days and after that period I fail to run a successful PCR . How can I elevate the life of primers I recently change my company an ordered a new one but unfortunately the problem has persist   to me yet. I work with ITS related primers in parallel but never faced such problem.

PS: I used DNA free water to wet my primers as company ordered.  

سلام آقاي نجاري

من هم براي استخراج دي ان اي از نمونه هاوي شفاف شده با لاكتوفنول مشكل دارم. شما از اين روش پيشنهاد شده استفاده كرديد؟ آيا جواب خوبي گرفتيد؟ 

با تشكر

Please search online protocol for extraction of DNA from phenolic samples 

you shold add other material to your lysate buffer to eleminate

Extera phenole.the last protocol was not fair to my extraction.

thank you a lot