The phenolic compound content from extraction optimization of a herbal plant was 7.38 mg GAE/g. Some posts here recommended that the scatter plot is established by concentration dilution series and % inhibition. So I will take my original extract ( phenolic content of 7.38 mg/g) to make 2, 3, 4, 5, 6 fold dilutions? Then I can calculate % inhibition through absorbance measurement . Afterward I can get IC50 from equation of straigh line.
Is my procedure right to determine antioxidant capacity expressed as IC50 value ?
Also check this Thi.
Regards
https://www.mdpi.com/1420-3049/25/24/6005/pdf
Dear Thi Van Thanh Do thanks for posting thie interesting technical question on RG. In my experience it is often quite useful to check the answers given to closely related technical questions which have been posted earlier on RG. In this context please have a look at the following questions:
How do i use DPPH assays to determine antioxidant activity of plant extracts?
https://www.researchgate.net/post/how_do_i_use_DPPH_assays_to_determine_antioxidant_activity_of_plant_extracts
(4 answers)
and
How do I calculate the IC 50 value of an extract and standard using a DPPH assay?
https://www.researchgate.net/post/How_do_I_calculate_the_IC_50_value_of_an_extract_and_standard_using_a_DPPH_assay
(10 answers)
Good luck with your work and best wishes!
Dear Thai Van,
The point in the preparation of the series is that you need to prepare a series that will give inhibition % close to both ends (0 and 100). you should start with the original concentration that give inhibition % close to 100, then prepare a series by making double volume dilutions.
However, the plot will not give a straight line ever.
If you know how to use softwares like the graphpad prism and the likes, you can plot your %inhibition against concentration graph and generate the IC50 values using the software. It's not that difficult.
And please do check out the earlier submissions/links as well.
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