Can a multi-junction solar cell still exceed the SQ-limit of single junction solar cells, if all its junctions were connected in parallel?

In a multi-junction solar cell, individual cells are stacked upon each other. But while the (theoretical) limiting conversion efficiency scales with the number of series-connected sub-cells, this...

17 March 2020 404 3 View

Why does my empty vector control fluoresce more than my fluorophore-containing vectors?

I'm using the SpectraMax M3 from Molecular Devices to read 96 well plates which contain wells with E. coli transformed with vectors containing a fluorophore under an inducible promoter. On this...

03 November 2019 1,787 1 View

Could anybody help me? What is the problem with my protocol of lymphocyte separation? Or our patients have got too high red blood cell number?

In general the separation works without problem, but sometimes the reaction is not convenient.I think the red blood cell get stuck in the gradient. If I tried to discharge the cell layer, I...

13 July 2016 2,435 14 View

Does anyone know a good tutorial for using R in mass spec data sets?

I want to manage and analyse my data sets from mass spec experiments with the program R. I'm just a beginner in R, so I would be happy for any suggestions.

27 October 2012 4,052 3 View

Do you think ACT-based group therapy can be augmented with computer- and app-support?

Visit our site for some concepts! Best Raphael

01 January 1970 334 2 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

In methylene blue test Why is UV absorbance reading very low as soon adsorbent is added and then increasing before reaching equilibrium?

Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...

19 February 2021 472 5 View

Has anyone performed a colorimetric RT-LAMP assay using Lucigens LavaLAMP kit?

Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...

17 February 2021 5,399 1 View

Passivation of chromium cast Iron in high temperature?

14 February 2021 2,157 3 View

Does anyone know if the Quantstudio 3 qPCR machine requires a passive reference when Using SYBR? I ran a qPCR and got the plot in the attached file.?

I know that my target is in my sample as I extracted it from cell culture myself. These plots show some amplification. The first one seems O.K for the first 36 cycles but then collapses at the...

14 February 2021 2,809 2 View

Why stop the reaction in ice for the fluorescent probe FDA (fluorescein diacetate)?

Hi, In literature I noticed that who stains haemocytes with FDA for 30 min then stop the reaction in ice while this does not apply for 15 min incubation time (or maybe authors did not write this...

08 February 2021 3,366 3 View

What is the possible cause of MSCs low growing?

Hello Everyone, We are inexperienced in culture of MSCs trying to grow a sample from BM of a mare. It is the second time that after preparation of new medium (DMEM-F12 with high glucose - 10% Hy...

07 February 2021 9,989 7 View

Why do cells die after adding MTT dye?

I was doing MTT assay, and in the last day before adding the MTT; the cells looked good (or so I think), but after aspiration of the drug and addition of MTT dye I noticed that most cells seemed...

07 February 2021 3,846 4 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View